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Journal: Investigative Ophthalmology & Visual Science
Article Title: YBX1 Modulated Corneal Neovascularization Induced by Alkali Burn via m 5 C-Dependent Regulation of the STAT3/HIF-1α/VEGFA Axis
doi: 10.1167/iovs.67.2.42
Figure Lengend Snippet: YBX1 regulates angiogenesis and apoptosis in HUVECs through the HIF-1α pathway. ( A–H ) Western blot results and quantification of HIF-1α, VEGFA, Bcl-2, Bax, cleaved PARP, and cleaved Caspase-3 expression levels after overexpression of HIF-1α in the context of YBX1 knockdown. ( I ) TUNEL staining of control, H/R and different treatment groups evaluating by fluorescence microscope. Magnification = 40×; Scale bar = 50 µm. ( J ) Double ICC labeling for VEGFA and CD31 in HUVECs with YBX1 knockdown and HIF-1α overexpression. Magnification = 400×; Scale bar = 50 µm. ( K ) Scratch wound healing assay showing changes in cell migration ability among different treatment groups. Magnification = 100×; Scale bar = 500 µm. ( L ) Transwell migration assay assessing invasion capacity after YBX1 knockdown and HIF-1α overexpression. Magnification = 400×; Scale bar = 125 µm. * P < 0.05 versus the control group, # P < 0.05 versus the H/R group, ◆ P < 0.05 versus the H/R + siNC group, & P < 0.05 versus the H/R + siYBX1. Data are presented as mean ± SEM ( n = 6). Means were compared by 1-way ANOVA with Tukey's multiple comparison post hoc tests.
Article Snippet: Cell apoptosis was assessed via
Techniques: Western Blot, Expressing, Over Expression, Knockdown, TUNEL Assay, Staining, Control, Fluorescence, Microscopy, Labeling, Wound Healing Assay, Migration, Transwell Migration Assay, Comparison
Journal: bioRxiv
Article Title: In vivo vaccine generation with a topical small molecule cocktail to eradicates AML and PDAC
doi: 10.1101/2025.10.19.683274
Figure Lengend Snippet: A . Representative and the corresponding local enlarged images of H&E, TUNEL (Green), CD11b (Red), Ly6G (Cyan), Ly6C (Magenta) staining of mice tumor from each treatment group, counterstained with DAPI (Blue). B-E . Quantification of TUNEL positive ( B ), CD11b + ( C ), Ly6G + ( D ), Ly6C + ( E ) cells in tumor tissue (n=3). F . Representative (left) and the corresponding local enlarged (right) images of H&E and CD11b (Red) staining of mice tumor from naïve and vaccine-cured mice tumor, counterstained with DAPI (Blue). G . Quantification of CD11b + cells in tumor tissue (n=3). All error bars represent means ± s.e.m. Statistical significance was determined by one-way ANOVA. * p<0.05, ** p<0.01, *** p<0.001, **** p<0.0001, ns means no significance.
Article Snippet: The
Techniques: TUNEL Assay, Staining